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Safflower yellow can significantly inhibit ADP-induced platelet aggregation in rabbits, and also has a very obvious depolymerization effect on ADP-aggregated platelets. When the dosage was 0.22g/ml, the inhibition rate of aggregation and depolymerization reached 85.9% and 78.9% respectively. These effects of safflower yellow were enhanced with the increase of dosage. Safflower yellow has a very significant inhibitory effect on experimental thrombosis in rats, the inhibition rate is 73.4%. Because the thrombus material formed on the silk line is platelet aggregates, the reduction of wet weight of thrombus is obviously the result of drug inhibiting platelet aggregation. It is consistent with in vitro experiments that safflower yellow can inhibit platelet aggregation induced by ADP. Safflower yellow also significantly prolonged plasma recalcification time, prothrombin time and coagulation time in rabbits. It shows that it can affect the coagulation system both in vivo and in vitro. In addition, safflower oil can reduce blood lipid.